Journal: Molecular pharmaceutics

Article Title: Camptothesome Potentiates PD-L1 Immune Checkpoint Blockade for Improved Metastatic Triple Negative Breast Cancer Immunochemotherapy

doi: 10.1021/acs.molpharmaceut.2c00701

Figure Lengend Snippet: (a) Flow cytometry investigation of the apoptotic and necrotic 4T1 cells by FITC Annexin V and propidium iodide (PI) after 20 h of treatment with free CPT, Onivyde® and Camptothesome at 4 μM or 15 μM of equivalent (eq.) CPT. Lipo-SM/Chol was used at the eq. SM as used in Camptothecome. (b) Frequency of early apoptotic (Annexin V+ PI−) and necrotic/late apoptotic (Annexin V+ PI+) 4T1 cells. (c) Flow cytometry determination of the calreticulin expression on 4T1 cells induced by free CPT, Onivyde® or Camptothesome for 20 h at 4 μM or 15 μM of CPT or irinotecan, respectively. (d) Normalized calreticulin expression ratio as compared to no treatment control. (e, f) The released ATP (e) and HMGB-1 (f) concentrations in the media of samples from (d), respectively. Data are expressed as mean ± s.d. (n = 3). Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: After the cells been trypsinized and collected, the cells were washed with cold PBS twice then stained with the FITC Annexin V apoptosis detection kit (BD Pharmingen ™ , #51-65874X) and PI (BD Pharmingen ™ , #51-66211E) according to the manufacturer’s instructions.

Techniques: Flow Cytometry, Expressing

Journal: Heart rhythm

Article Title: Biomarkers to Predict Improvement of Left Ventricular Ejection Fraction after Atrial Fibrillation Ablation.

doi: 10.1016/j.hrthm.2024.04.044

Figure Lengend Snippet: Figure 1: A: Receiver operator curves of a Biomarker Panel (ANG2, GDF 15, FGF23, MyBPC3) B: Receiver operator curves of the 534

Article Snippet: ANG2 Human Angiopoietin2 / ANG2 ELISA Kit PicoKineTM 5.2$ https://www.bosterbio.com/ 2.

Techniques: Biomarker Discovery

Journal: International Journal of Clinical Practice

Article Title: Salivary Histatin 5 Level in Women with Vaginal Candidiasis

doi: 10.1155/2022/5279323

Figure Lengend Snippet: SDS-PAGE profiles of salivary samples. Lanes represented by 1 to 35 indicate patient and control. M represents protein marker (Biorad 1610377). H represents histatin 5 standard (MyBioSource MBS826319).

Article Snippet: The Human Histatin 5 ELISA Kit (E1444 Hu, BT Lab, Shanghai, China) was used.

Techniques: SDS Page, Control, Marker

Journal: Translational Oncology

Article Title: Defective transcription elongation in human cancers imposes targetable proteotoxic vulnerability

doi: 10.1016/j.tranon.2021.101323

Figure Lengend Snippet: TE def cells contain high levels of insoluble protein aggregates (aggresomes): A. Confocal images show aggresome puncta in control (black) versus transcriptionally defective (red) breast cancer cell lines stained with Proteostat reagent as indicated. The right panel represents magnified views of the white boxed regions, scale bars; 20 µm; B. Quantification of aggresome puncta volume calculated from 3D Z-stack confocal images in A , mean values of two independent experiments are shown; C. Immunoblots show insoluble ubiquitinated protein aggregates in the insoluble fraction of the TE def lysates; D. Densitometry quantification of the ubiquitinated protein aggregates in the insoluble fraction of C with fold changes normalized to UACC812; E. For each gene, the reads in the soluble and the insoluble fractions were pooled per cell line, and the proportion of reads in the insoluble fraction over total was calculated (0 meaning all the reads for that gene came from the soluble fraction, and 1 meaning that all the reads for that gene came from the insoluble fraction). The boxplot here shows the distribution of these proportions for all the genes in each sample, showing a significant enrichment in the TE def cells. F. For each gene, differential expression using t -test was calculated between TE def and TE prof cells in the soluble and insoluble fractions. The barplot shows the number of genes that were found to be significantly ( P < 0.05) increased in TE def cells in the soluble and insoluble fractions. More genes were found increased in the insoluble fraction in TE def cells G. GO-Elite was used to analyze the gene ontologies of differentially expressed genes that were enriched in the insoluble fraction of TE def cell lines compared to TE prof cell lines. **= p <0.01.

Article Snippet: Aggresomes and misfolded proteins were observed with Proteostat Aggresome Detection Kit (Enzo Life Sciences, Farmingdale, NY, US, ENZ-51,035-K100) following the provider's instruction.

Techniques: Staining, Western Blot, Expressing